This comes as no surprise, since amla has been prized for centuries for its numerous healthful properties. Amla Emblica officinalis is a glossy green spherical fruit with a distinctive astringent taste that grows wild in the tropical forests of India, as well as in the tropical and subtropical areas of China and Myanmar.
The contributions of the plants are enormous in every sector of human life. It helps to growing up of the human body and also protects human being from sickness by being used as medicine. A large number of plants are used as medicinal agents in this world. Specifically in Bangladesh about two hundred fifty species are used as medicinal plants 1.
It has now been established that the plants which naturally synthesize and accumulate some secondary metabolites like alkaloids, glycosides, tannins, volatiles oils and contain minerals and vitamins possesses medicinal properties 2.
Recent trend is to integrate the traditional medicine with modern medicine. Best therapeutic results are said to be obtained often with the traditional Chinese system 3.
The drug resistance of human and animal pathogens is one of the best documented cases of biological evolution, and is a serious problem both in developed and developing countries. The daily consumption of more than one ton of antibiotics in some countries has resulted in resistance to bacterial populations, thus causing a serious public health problem.
The fruit is drupe, fleshy globose, 1. It is distinctly marked in six lobes. The fruit is green when tender but the colour changes to light yellow or brick red on maturity.
The taste is sour and astringent giving feeling of sweetness afterwards 5. The plant is found in the mixed deciduous forests of India, Sri Lanka, China, Bangladesh and Malaya ascending to 1, metres on the hills 6. Dried fruit is useful in haemorrhage, diarrhea, diabetes and dysentery.
The fruit has antibacterial, antifungal, and antiviral activities 7. This plant has been chosen for the present investigation because of its availability and wider indication in various diseases.
After complete drying, the fruit samples were ground into coarse powder with the help of a mechanical grinder and the powder was stored in a suitable container for extraction process.
Preparation of the Plant Extract: The powdered material was successively extracted with ethanol and acetone by using cold extraction process 8. At first gm of dried powder was taken in an aspirator 5L. Before placing powders into the aspirator, the jar was washed properly and dried.
The time duration was of 21 days at room temperature with occasional shaking and stirring for each successive extraction.
It was then filtered through a fresh cotton plug and finally with a Whatmann filter paper no. In the same way the powdered material was extracted with acetone. Antibacterial and Antifungal Assay: Ten pathogenic bacteria and fungi were used as test organisms for antibacterial activity of the dried extracts.
Both extracts were tested for antimicrobial study by using standard disc diffusion method 9, Nutrient agar media was used for culture of the test organisms and the antibacterial and antifungal activities were determined by single disc diffusion method Then from this clear solution concentrated agar plates were prepared.
The standard discs 0. The sample discs, the standard antibiotic discs and the control discs were placed gently on the previously marked zones in the agar plates, pre-inoculated with test organism.
The discs were then incubated on the plate aerobically at 37oC for 24 hours. The diameter of inhibition zone around each disc was measured and recorded at the end of the incubation period. The extract concentration able to inhibit microbial growth, which was observed through the formation of an inhibition growth zone around the disc equal to or greater than 8 mm 12was considered.
In vitro Antibacterial Study:ON EMBLICA OFFICINALIS Research problems pertaining to these drugs Attempts have been made to investigate some of the drugs mentioned by Avicenna (2,3,4).
This paper deals with one such drug namely Emblica officinalis. The fruit pulp of Emblica officinalis (English: Embelic Myrobalans: Hindi: Amla/Amalaki.
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a review on medicinal importance of emblica officinalis html full text. a review on medicinal importance of emblica officinalis. r. jain *, r. pandey, r. n.
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1 Department of Pharmacy, Sagar Institute of Technology & Management, Barabanki, Uttar Pradesh, India. 2 S. D. College of Pharmacy and .